Flow cytometry is used for cell analysis and is focused on measuring protein expression or co-expression within a mixed population of cells. What is the difference between FACS and flow cytometry?įACS is used as a cell sorter and enriched for a subset of cells which is often then studied in further detail using flow cytometry or other analytical techniques2. This method may be used to evaluate cells from blood, bone marrow, body fluids such as cerebrospinal fluid (CSF), or tumors. This method can also identify particular components within cells. What can flow cytometry detect?įlow cytometry is a laboratory method used to detect, identify, and count specific cells. How do you represent flow cytometry data?įACS data are commonly presented as one- dimensional histograms or two-dimensional displays (dot displays or contour maps) with logarithmic axes that extend over a 'four- to five-decade' range, representing cells with flourescence values that differ 10,000- to 100,000-fold between the lower and upper ends of the scale.
Cell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths. The basic principle of flow cytometry is the passage of cells in single file in front of a laser so they can be detected, counted and sorted. Flow cytometry studies are used to identify and quantify the cells of the immune system and to characterize hematological malignancies. What is the purpose of flow cytometry?įlow cytometry provides a well-established method to identify cells in solution and is most commonly used for evaluating peripheral blood, bone marrow, and other body fluids. The population then flows past a set of laser light sources one cell at a time. The suspension is funneled through a nozzle that forges a single-cell stream. Flow cytometry is a method of single-cell analysis that includes the characterization of a cell's physical properties.
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